Quantification of total phenolic, flavonoid content and HPTLC fingerprinting of Senecio laetus

Abstract

Senecio laetus was chosen for this study because it lacks data, making it necessary to carry out its phytochemical screening, total phenolic and flavonoid content analysis, TLC and HPTLC fingerprinting, and establish the standard pharmacognostical parameters for this plant. The quality and purity of the plant species can be determined with the use of these factors.  To find several phytoconstituents, preliminary qualitative phytochemical screening was conducted. Additionally, the contents of phenolic and flavonoid compounds were quantified. Also, methanolic extract HPTLC fingerprinting was carried out. Different fractions contained carbohydrates, alkaloids, glycosides, saponins, flavonoids, tannins, phenols, etc., According to preliminary phytochemical analysis. With 785.52 mg/g of GAE and 202.33 mg/g of RE, respectively, ethyl-acetate has the highest phenolic and flavonoid content among all the fractions. Alcoholic extract's HPTLC fingerprinting revealed seven polyvalent phytoconstituents.